Genetic and Molecular Evaluation of Newly Selected Tomato Lines for TYLCV Resistance
Keywords:
Tomato, TYLCV, Genetic parameters, Pedigree selectionAbstract
Fifteen new lines of tomato were developed from three different hybrids for resistance to tomato leaf curl virus (TYLCV) with desired yield, vegetative, and earliness traits. The 15 lines were evaluated at the 6th generation first under artificial infection conditions and then under field conditions. The evaluated traits were plant length (cm) and number of plants. of branches/plant, first flowering, days to first harvest, no. of fruits\ plant, weight of fruits/plant, and total yield/plant (kg). The results indicated that the genetic variance was positive and higher than the environmental variance for all studied traits. These results were confirmed by the values of heritability, which ranged from 54.42 to 90.26 for No. of branches and Days 1st Flowering, respectively. Line 3 recorded the earliest flowering and days to first harvesting with mean values 17.35±0.88 and 65.0±0.40, respectively. While Line 4 recorded highly mean values for the number of fruits/plant and total yield/ plant, with mean values 31.3±1.24 and 3.28±0.13, respectively.
Among the selected lines, one line was Immune/Highly Resistant (HR), six lines were resistant, and seven lines showed moderate resistance to TYLCV. This finding was confirmed by marker-assisted selection, where Ty-1 and TY3 showed that Lines (7, 8, 9, 10, 11, 12, and 13) were dominant homozygous for ty1; in addition, lines 14 and 15 were dominant homozygous for ty3, and lines 3, 4, and 5 were heterozygous for Ty3. From these results, it could be concluded that the pedigree selection procedure is highly effective in selecting promising resistant tomato lines for TYLCV.
References
Accotto, G.P.; J. Navas-Castillo; E. Noris; E. Moriones and D. Louro (2000).Typing of tomato yellow leaf curl viruses in Europe. European Journal of Plant Pathology, 106: 179–186.
Allard, R.W (1999). History of Plant Population Genetics. Annual Review of Genetics, 33: 1-27.DOI.org/10.1146/annurev.genet.33.1.1.
Allard, R. W. (1960). Principles of Plant Breeding. John Wiley & Sons, New York.
Babu, M. R.; R. K. Reddy;A. Rani.; and P. Saidaiah (2018). Genetic improvement for yield, quality and leaf curl virus resistance in tomato (SolanumLycopersicumL.) Journal of Pharmacognosy and Phytochemistry, 1048-1055.
Dar, RA.; and J.P.Sharma (2011). Genetic variability studies of yield and quality traits in tomato (Solanum Lycopersicum L.). Int. J Plant Breeding Genet, 5 (2):168-174.DOI: 10.3923/ijpbg.2011.168.174.
Dellaporta, S.L.;J. Wood and J. B.Hicks (1983.). Plant Molecular Biology Reporter, 4: 19-21.
Diaz-Pendon, J.A.; M. Carmen ; E. Moriones; E. R begarano; E.D uardo;H. Czonek H ;and J .N-Castillo (2010). Tomato yellow leaf curl viruses: ménage ‘a trois between the virus complexes the plant and the whitefly vector. Plant Molecular Biology 1: 19-21. Plant Molecular Biology, 1: 19-21DOI:10.1111/j.1364-3703.2010.00618.x.
El-Morsy, A, S.; M.I. Mahmoud; A.I. El-Kassas; and A.M. Kansouh(2021). SElection and Breeding New Lines of Tomato (Solanum Lycopersicon L.) Resistance to Tomato Yellow Leaf Curl Virus. SINAI Journal of Applied Sciences,10 (2): 99-106.
Garcia , A .A .; E: E. Graham:, S A Brown, K Kouzekanani; and C. L. Hanis (2001). The Starr County Diabetes Education Study: development of the Spanish-language diabetes knowledge questionnaire Diabetes Care,24(1):16-21. DOI: 10.2337/diacare.24.1.16.
Glick, E.; Y.Levy ; and Y. Gafni (2009). The viral etiology of Tomato Yellow Leaf Curl Disease A review, Plant Protection Science.45: 81–97.
Hanson, P.; S. K .Green and G. Kuo (2006). Ty-2, a gene on chromosome 11 conditioning geminivirus resistance in tomato. Tomato Genetics Cooperative Report ,56: 17-18.
Hutton, S. and J. W. Scott (2014). Ty-6, a major begomovirus resistance gene located on chromosome 10, Tomato Genetics Cooperative Report 64: 14-18.
FAO. (2023). FAOSTAT: Crops and livestock products. Food and Agriculture Organization of the United Nations. Retrieved from http://www.fao.org/faostat/en/#data/QC
Ji, Y. F.; J. Scott; P. Hanson.; E. Graham and D. P. Maxwell (2007). Sources of Resistance, Inheritance and Location of Genetic Loci Conferring Resistance to Members of the Tomato-Infecting Begomo viruses. Springer. Dordrechte, 343-62. DOI:10.1007/978-1-4020-4769-520.
Ji, Y; J. W. Scott; D. J. Schuster and D. P. Maxwell, (2009). Molecular mapping of Ty-4, anew tomato yellow leaf curl virus resistance locus on chromosome 3 of tomato. Journal of the American Society for Horticultural Science ,134: 281-288.
Koeda, S. and A. Kitawaki (2024). Breakdown of Ty-1-based resistance to tomato yellow leaf curl virus in tomato plants at high temperatures. Phytopathology, 114(1): 294-303. DOI: 10.1094/PHYTO-04-23-0119-R.
Kumar, A.; S. kgind; M.S. Dhaliwal; AS. Harma Jain and ST. Kaur (2019). Horticultural evaluation of advance preedinglines possessing differentCompensations of Ty and Ph genes in tomato (Solanum Lycopersicon L.). GENETIKA, No 3: 771-788, DOI.org/10.2298/GENSR1903771K.
Lapidot, M.; M. Friedmann; M. Pilowsky; R. Ben-Joseph and S. Cohen, (2001). Effect of host plant resistance to Tomato yellow leaf curl virus (TYLCV) on virus acquisition and transmission by its whitefly vector. Phyto pathology,91(12) :1209-1310.1094/PHYTO.2001.91.12.1209.
Macharia, I (2015). Pesticides and Health in Vegetable Production in Kenya. BioMed Research International, DOI.org/10.1155/2015/241516.
Marie, E. C. R.; J.Ndunguru; L.C Berrie; M.Paximadis;S. Berry; N. Cossa; V. N.Nuaila; G.K.Mabasa; N. Abraham; E.P. Rybicki; D. Martin; G.Pietersen; G. and L.L. Esterhuizen (2012).Diversity of Dicotyledonous-Infecting Geminiviruses And their Associated DNA Molecules in Southern Africa. Including the South-West Indian Ocean Islands. Viruses ,4: 1753-1791. DOI: 10.3390/v4091753.
Nogueira, D. G.;W. IR. Malufd;W. Nogueira Gabrie; M. Macie; l. L.V. Paivae and A.D .R. Figueira (2001)Microsatelite marker associated with the Ty 1 allele for resistance to Begomovirus in tomato. Pesquisa Agropecuária Brasileira, DOI: 10.1590/S0100-204X2011000400011.
Patel, M.S.; N. Singh; A. Kumar; M.K. Singh; G.C Yadav; And M.B.Ghuge(2015). Genetic Variability, heritability and genetic advance of growth and yieldcomponents of tomato (Lycopersiconesculentum M.). Environ. and Ecol., 33 (3): 1034-1037.
Picó, B.; M. Ferriol; M.J. Diez; and F. Nuez (1999). Developing tomato breeding lines resistant to tomato yellow leaf curl virus Plant Breeding, 118 (6): 537-542.
Prasad G. S. V.; G. Padmavathi; K. Suneetha; M. S. Madhav; and K. Muralidharan (2020). Assessment of diversity of Indian aromatic rice germplasm collections for morphological, agronomical, quality traits and molecular characters to identify a core set for crop improvement. CABI Agric Biosci, 1:13. doi.org/10.1186/s43170-020-00013-8.
Prasanna, H. C.; D. P. Sinha; G. K. Rai; R. Krishna; S. P. Kashyap; N. K. Singh;M. Singh and V. G. Malathib(2015). Pyramiding Ty-2 and Ty-3 genes for resistance to monopartite and bipartite tomato leaf curl viruses of India. Plant Pathology ,64. :256–264. DOI: 10.1111/ppa.12267.
Rai AK.; A. Vikram and A. Pandav (2016). Genetic variability studies in tomato (Solanum Lycopersicon L.) for yield and quality traits. International Journal of Agriculture, Environment and Biotechnology, 9 (5):739-744.
Ravindra, V.; S. S. Senglaub; A. Rattani; M.C. Dewan; R. Hartl; E. Bisson; K. B. Park and Park. G. Shrime (2018). Degenerative Lumbar Spine Disease. Estimating Global Incidence and Worldwide volum. Global Spine Journa, 8(8): 784-794. DOI: 10.1177/2192568218770769 .
Samarakoon,S.A. M.C.; A. Balasuriya; R.G.A.S Rajapaksha; and W.A.R Wickramarachchi.( 2012).Molecular detection and partial characterization of Tomato Yellow Leaf Curl Virus in Sri Lanka. Pak J Biol Sci, 15;15(18):863-70.
Samima, Y .; P. Dukpa; D. Maurya ;V. Jatav; and S. Dwary (2023).Genetic variability, heritability and genetic advance for yield and yield contributing traits in Tomato (Solanum Lycopersicum L.) .International Journal of Advanced Biochemistry Research, 7(2):207-211. DOI:10.33545/26174693.2023.v7.i2Sc.214.
Siam Joy,I.; Rocky; S, Sultana; M. Kumkum; and B. Hossain(2025). Evaluation of Selected Tomato Cultivars effectiveness against Tomato Yellow Leaf and Its PCR-based molecular detection. European Journal of Agriculture and Food Sciences, 2684-1827. http://dx.doi.org/10.2 4018/ejfood.2025.7.2.898.
Saikia, A. K., & Muniyappa, V. (1989). Epidemiology and control of Tomato Leaf Curl Virus in southern India. Tropical Agriculture, 66(4), 350-354.
Singh R.K.; and B.D. Chaudhary (1985). Biometrical Method in Quantitative Genetics Analysis. Kalyani Publishers, New Delhi., CABI Record Number: 19801689021.
Sivasubramanian S. and P. Madhavamenon (1973). Combining ability in rice. Madras. Agric, J.;60:419-421.
Verlaan, M. G., S. F Hutton., R. M. Ibrahem., R. Kormelink., R. G. F Visser., J W.Scott., J. D. Edwards; and Y Bai (2013). The Tomato Yellow Leaf Curl Virus resistance genesTy-1 and Ty-3 are allelic and code for DFDGD-Class RNA–dependen t-RNA Polymerases. Genetics, https://doi.org/10.1371/journal.pgen.1003399.
Vijeth, S.; M. S. Dhaliwal; S. K. Jindal and A. Sharma(2018). Evaluation of tomato hybrids for resistance to leaf curl virus disease and for high‑yield production. Horticulture, Environment, and Biotechnology, 59:699–709doi.org/10.1007/s13580-018-0080-5.
Yan, Z. ;A . M. A. Wolters; J. N. Castillo; and Ybai (2021). The Global Dimension of Tomato Yellow Leaf Curl Disease. Microorganisms, 9- 740. https://doi.org/10.3390/microorganisms9040740.
Yang Y. ; N. S .Rao; E Walker; S Sen ; and J. Qin (1997) . Nuclear magnetic resonance assignment and secondary structure of ankyrin-like repeat-bearing protein: myotrophin. Protein Sci, 6(6):1347-51. DOI: 10.1002/pro.5560060625.
YuanfuJ.: J. David: Schuster:J. W. Scott (2009). Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato. Mol Breeding, DOI: 10.1007/s11032-007-9089-7.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 WSEAS Transactions on Biology and Biomedicine
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Author(s) and co-author(s) jointly and severally represent and warrant that the Article is original with the author(s) and does not infringe any copyright or violate any other right of any third parties and that the Article has not been published elsewhere. Author(s) agree to the terms that the WSEAS Journal will have the full right to remove the published article on any misconduct found in the published article.
